
Molecular mechanisms of DNA repair, replication, and mutagenesis. Biochemical, biophysical and cellular studies of dynamic protein-DNA interactions. We are interested in basic mechanisms and abnormal mechanisms associated with cancer, immunodeficiency, and aging.

pathogenesis, cryo-EM, structural biology, membrane proteins

The organization of intracellular components is dependent on the microtubule (MT) cytoskeleton, a network of rod-like filaments that form and disassemble on a rapid timescale. During cell division, MTs assemble into the mitotic spindle, where they generate forces that move chromosomes. MTs are also essential for cytokinesis, where they facilitate cleavage plane specification, constriction of the cleavage furrow, and abscission. The R. Ohi laboratory uses live cell imaging and reconstitution biochemistry to understand how MTs are organized and remodeled during cell division. Over the past...

virus assembly; virus entry; protein trafficking; protein-RNA interaction; protein-membrane interaction

Role of keratin proteins in epithelial differentiation and epithelial homeostasis