CRISPR Screening, LDL uptake
tissue engineering, reproduction, biomaterials
Research in the Singer lab is focused on understanding the influence of diet-induced obesity on hematopoiesis and the generation of activated macrophages that lead to metabolic disease.Current projects in the laboratory focus on (1) sexually dimorphic inflammatory responses responses to high fat diet and (2) mechanisms driving hematopoietic stem cell myeloid differentiation after high fat diet exposure. This work in mouse models uses bone marrow transplantation, stem-cell analysis techniques, and metabolic profiling.
We investigate biological function and mechanism at the molecular level by developing a structure-based understanding of proteins and macromolecular complexes. The lab studies biosynthetic pathways for natural products, proteins of pathogenic viruses, and host proteins that defend against viral pathogens.
G protein coupled receptors (GPCRs) form a large family of cell surface receptors responsible for triggering cellular responses to a variety of extracellular stimuli including drugs such as opiates, and hormones such as adrenaline, serotonin or acetylcholine. We focus on analysis of G protein signal transduction pathways at a molecular, cellular and translational level with the goal of understanding how these pathways control physiology and disease. We use a broad range of approaches including biochemistry, molecular biology, live cell imaging technologies and cutting edge genetic...
The Role of Mitophagy in Diabetes
All forms of diabetes share the common etiology of insufficient insulin release from pancreatic islet beta cells to meet peripheral insulin demand. Beta cells require mitochondrial function in order to maintain proper glucose stimulated insulin release. Our lab focuses on the molecular and genetic regulation of the mitochondrial life cycle, with a focus on mitophagy, a pathway to dispose of unhealthy or damaged mitochondria. Our studies also focus on novel genetic targets affecting the mitophagy pathway, which are also associated with diabetes in...
The Speers laboratory is interested in “bench to bedside” research that includes basic mechanistic studies, translational pre-clinical studies, and clinical research. As PI or co-Investigator on several university-, industry-, private foundation- and NIH-funded grants, we remain active in the radiation and breast cancer research arena by looking for more effective, targeted therapies for women with breast cancer. These targeted therapies include PARP-inhibitors, CDK 4/6 inhibitors, and androgen receptor antagonists as agents for radiosensitization. We have also utilized kinome screens to...
virus-cell interaction, positive-strand RNA viruses, flaviviruses, cell biology of RNA virus infeciton
G protein coupled receptors (GPCRs) activate heterotrimeric G proteins to control myriad signaling pathways and physiological processes. Our lab broadly studies these signaling circuits with current emphasis on delineating the function of adhesion GPCRs and Ric-8 proteins (folding chaperones for all G protein alpha subunits). Adhesion GPCRs appear to couple extracellular shear force events to intracellular G protein signaling. We use a wide range of approaches, including purified protein structure/function, cell culture models, GPCR drug screening, and transgenic animal models.
We study nucleotide repeat expansions as both mediators of normal neuronal function and drivers of neurologic diseases such as Fragile X Syndrome, FXTAS, ALS, and Frontotemporal Dementia. We use Drosophila, human neurons, biochemical approaches and next gen sequencing to define the mechanisms by which repeats cause disease and develop novel therapeutics. Recent work has focused on both alternative modes of protein translation and the biology repeat containing RNAs. Visit our Lab Webpage.
Our laboratory is interested in elucidating the molecular mechanisms underlying chromatin modifications that regulate gene expression and other chromatin-associated functions. Our studies are primarily focused on the enzymes that dynamically control the methylation status of lysines in histones and non-histone proteins using structural and biochemical approaches.
The Truttmann laboratory studies molecular chaperone functions in the context of proteostasis, aging and aging-associated diseases (e.g. Alzheimer’s disease, Huntington’s disease, Ataxias, etc.). We are particularly interested in the regulation and functions of heat shock protein 70 (Hsp70) family proteins (e.g. HSC70, Grp78/BiP, etc.). We employ numerous genetic, biochemical as well as behavioral approaches in conjunction with several model systems (tissue culture, Caenorhabditis elegans, mice, primary human tissue) to address our research questions in a holistic fashion.
The Tsai laboratory is interested in clarifying the cellular entry pathways of a bacterial toxin (cholera toxin) and a DNA tumor virus (polyomavirus). Specifically, we wish to identify the host factors hijacked by these toxic agents during the course of infection, and to illuminate the molecular nature of the host-pathogen interactions.
Aging and stress response pathways
Protein aggregation dynamics in C. elegans polyglutamine disease models; Behavior and function of physiological polyglutamine-containing proteins
Investigating the mechanism behind recognition/regulation of modified microtubules using cryo-EM.
The aims of our research are to understand the core mechanisms that drive brain cancers such as diffuse intrinsic pontine gliomas( DIPG) and ependymomas in children, as well as gliomas in adults. With this knowledge we aim to develop treatments and cures for these deadly tumors.We study the relationship between cancer metabolism and epigenetics in brain tumors. Oncogenes in primary brain tumors in adults and children reprogram uptake and metabolism of nutrients such as glucose and glutamine to modulate many cellular functions including epigenetics. We evaluate the effects of altered...
cytoskeleton, intracellular trafficking, microtubules, kinesin motor proteins, cilia
The mechanisms of myelin targeting in the inner ear.
The Walter group studies the cellular and molecular biology of ubiquitous non-coding RNAs, composing perhaps 90% of the human genome and broadly responsible for the regulation of gene expression, by mechanistic single molecule and super-resolution fluorescence microscopy tools inside and outside of live cells. Applications of this leading-edge, highly interdisciplinary work include the identification and optimization of novel RNA-targeting drugs for gene therapy and of RNA and DNA as diagnostic biomarkers.
Uncovering the mechanisms of serotonin-based signaling in food perception and DR-mediated longevity.
We seek to understand essential cellular processes — using cultured neurons, cell lines and yeast — in order to shed new light on neurodegeneration, cancer and other diseases.
Normal as well as neoplastic cell populations remodel the extracellular matrix by regulating the expression of complex gene programs that control cell motility, proteolytic activity, proliferation and morphogenesis. However, the identity of the upstream and downstream gene products that regulate these activities in normal or pathologic states remain unclear, especially within the context of the 3-dimensional matrix. Current efforts focus on identifying the regulation of transcription factors that control epithelial-mesenchymal transitions in carcinomatous states as well as the genetic...
Our lab profiles the mechanisms by which multi-nucleated osteoclasts stimulate osteoblastic bone formation in order to identify novel therapeutic targets to promote bone formation in aging and cancer-induced bone disease. We use cell culture and animal models to dissect out osteo-immunology signaling pathways differentially regulated in osteoclast resorption and stimulation of bone formation. We are working across disciplines (engineering, materials science, pharmacy, medicine) to develop novel tools to study resorption and single cell osteoclast dynamics.
Cardiac myocyte; Sarcomere; Post-translational Modifications; Protein kinase C
DNA repair, mutagenesis, transcription, genomics, bioinformatics
virus-host interactions in the intestine, norovirus, astrovirus, human intestinal organoids
cancer biology, stem cell biology, skin, epithelial tissue
Mechanism of neurodegeneration in Amyotrophic Lateral Sclerosis (ALS)
The current research in our laboratory is focused on ion channels in the lysosome and their roles in the pathogenesis of lysosome storage diseases, metabolic diseases, and common neurodegenerative diseases. We use an integrative approach with state-of-art techniques including molecular biology, bioinformatics, biochemistry, immunochemistry, electrophysiology, fluorescence imaging, spinning-disk confocal microscopy, mouse genetics, and genome editing technologies.
Neuron development, neural circuits, neurological disorders, Down syndrome, Drosophila and mice